치과용 Ni-Cr합금 용출배지에 의한 인간 피부 섬유아세포 성장도 관찰 연구
A Study on the Viability of Human Dermal Fibroblast Cell by Media for Ni-Cr alloy elution
김갑진, 김치영, 최성민,
소속 상세정보
김갑진 ( Kim Kap-Jin ) - (주)코리아덴탈랩
김치영 ( Kim Chi-Young ) - 부산가톨릭대학교 보건과학대학 치기공학과
최성민 ( Choi Sung-Min ) - 부산가톨릭대학교 보건과학대학 치기공학과
KMID : 0362120090310030021
Abstract
Purpose: Standards of alloy for porcelain fused to metal crown be classified by metallic factor and biological factor. Metallic factors consist of stability of alloy composition and mechanical strength and surface characteristics for chemical bond. Biological factors be considered properties of metallic elements and problems originated by toxicity and hypersensitive reaction. Alloys considered such controversial points are the most suitable alloy for dental instrument.
Method: Alloys added Be and Nb using Ni-Cr alloy which has been widely used for dental instrument be selected and classified experimental group. Non-addition Be and Nb to Ni-Cr alloy classify control group and addition Be alloy is Be-experimental group, addition Nb alloy is Nb-experimental group. Specimens for cytotoxicity analysis gave effect to washing and sterilization. and then made an experiment on elution with cell medium after disinfection. It conducted specimens within cell medium with 24hours, 48hours, 72hours, respectively. It cultured human dermal fibroblast(HDF) using cell medium for cytotoxicity test and then investigated elution rate through spectroscopic analysis by MTT-assay.
Result: As results of cytotoxicity test by MTT-assay, cultured cell rate of VII measured more low numerical value within elution medium for 24hours focused on control group. Also, cultured cell rate of K3 alloys observed low value for 48hours, 72hours than value of control group.
Conclusion: According to final result that synthesize above results, Ni-Cr alloy added Be and Ni has little difference in Cytotoxicity by MTT-assay.
키워드
Ni-Cr alloy;cytotoxixity;MTT assay;Human detmal fibroblast;HDF
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